ABSTRACT
Objective To estalish an accurate method for the determination of cinobufagin and resibufogenin in Jiuxin Tablet. Method Waters Symmetry Shield RP18 (3.9 mm?250 mm, 5 ?m) column in an oven at 30 ℃ was need, with a mobile phase consisting of acetonitrile-0.5mol/L kalium dihydrogen phospate (phosphoric acid preparating pH=3.2) (42∶58) and a UV detector at 296 nm, the flow rate was 1.0 mL/min. Results The linear ranges of Cinobufagin was 0.0696～0.8352 ?g. The RSD of measurement precision test was 0.53%, the average recovery was 101.86% (RSD=1.81%, n=5), the linear ranges of Resibufogenin was 0.1576～0.9456 ?g, the RSD of measurement precision test was 0.15%, the average recovery was 102.07% (RSD=1.62%, n =5). Conclusion The method was simple and accurate, and can be used for the quality control of Jiuxin tablet.
ABSTRACT
Objective To established a method for the quality standard of Shushenling Capsules. Methods Folium Perillae and Rhizoma Chuanxiong in Shushenling Capsules were identified by TLC. The content of emodin was determined by HPLC. Results The spots of samples on TLC can be well separated and the method had strong specificity. The averge recovery of emodin was 99.31% and RSD was 1.54%. Conclusion The method is simpie, sensitive and accurate. It can be used for quality control of Shushenling Capsules.